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KMID : 1098420110190030185
Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 3 p.185 ~ p.190
Development of SSR Markers for Identification of Korean Ginseng (Panax ginseng C. A. Meyer) Cultivars
Bang Kyong-Hwan

Chung Jong-wook
Kim Young-Chang
Lee Jei-wan
Jo Ick-Hyun
Seo A-yeon
Kim Ok-Tae
Hyun Dong-Yun
Kim Dong-Hwi
Cha Sun-Woo
Abstract
The principal objective of this study was to develop a discrimination method using SSR markers in Korean ginseng cultivars. Five cultivars--Chunpoong, Yunpoong, Gopoong, Sunpoong, and Kumpoong--were evaluated by nine markers out of 22 SSR markers. A total of 23 alleles were detected, ranging from 1 to 4, with an average of 2.6 alleles per locus, and an averages of gene diversity (GD) of 0.480. Nine markers were tested in order to distinguish among five Korean ginseng cultivars. Two markers out of nine SSR markers, GB-PG-065 and GB-PG-142, were selected as key markers for discrimination among Korean ginseng cultivars. Two genotypes were detected in GB-PG-065. Chunpoong and Kumpoong shared the same allele type, and Yunpoong, Gopoong, and Sunpoong shared another identical allele type. In the case of GB-PG-142, a specific allele type differentiated from those of other four cultivars was observed only in Sunpoong cultivar. Consequently, the SSR markers developed in this study may prove useful for the identification of Korean ginseng cultivars and the development of ginseng seed management systems, as well as tests to guarantee the purity of ginseng seeds.
KEYWORD
Korean Ginseng, Cultivar, Discrimination, Simple Sequence Repeats
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